Type

Journal Article

Authors

Timothy P Guinee
James A. O'Mahony
Alan L Kelly
Yingchen Lin

Subjects

Biochemistry

Topics
ethanol metabolism hydrogen ion concentration hot temperature micelles gels animals caseins chymosin chemistry milk

Addition of sodium caseinate to skim milk increases nonsedimentable casein and causes significant changes in rennet-induced gelation, heat stability, and ethanol stability. (2016)

Abstract The protein content of skim milk was increased from 3.3 to 4.1% (wt/wt) by the addition of a blend of skim milk powder and sodium caseinate (NaCas), in which the weight ratio of skim milk powder to NaCas was varied from 0.8:0.0 to 0.0:0.8. Addition of NaCas increased the levels of nonsedimentable casein (from ∼6 to 18% of total casein) and calcium (from ∼36 to 43% of total calcium) and reduced the turbidity of the fortified milk, to a degree depending on level of NaCas added. Rennet gelation was adversely affected by the addition of NaCas at 0.2% (wt/wt) and completely inhibited at NaCas ≥0.4% (wt/wt). Rennet-induced hydrolysis was not affected by added NaCas. The proportion of total casein that was nonsedimentable on centrifugation (3,000 × g, 1 h, 25°C) of the rennet-treated milk after incubation for 1 h at 31°C increased significantly on addition of NaCas at ≥0.4% (wt/wt). Heat stability in the pH range 6.7 to 7.2 and ethanol stability at pH 6.4 were enhanced by the addition of NaCas. It is suggested that the negative effect of NaCas on rennet gelation is due to the increase in nonsedimentable casein, which upon hydrolysis by chymosin forms into small nonsedimentable particles that physically come between, and impede the aggregation of, rennet-altered para-casein micelles, and thereby inhibit the development of a gel network.
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Full list of authors on original publication

Timothy P Guinee, James A. O'Mahony, Alan L Kelly, Yingchen Lin

Experts in our system

1
Timothy P. Guinee
Teagasc
Total Publications: 33
 
2
James A. O'Mahony
Teagasc
Total Publications: 34
 
3
Alan L. Kelly
Teagasc
Total Publications: 63