Type

Journal Article

Authors

S Fair
P Lonergan
S T Butler
L Brennan
A O'Gorman
H Whelan
C Murphy
B Fernandez-Fuertes
S A Holden

Subjects

Biochemistry

Topics
gas chromatography mass spectrometry pregnancy outcome amino acid thermal stress fatty acid flow cytometry glutamic acid pregnancy rate

Relationship between in vitro sperm functional assessments, seminal plasma composition, and field fertility after AI with either non-sorted or sex-sorted bull semen. (2016)

Abstract The hypothesis of this study was that different in vitro parameters are required to predict the in vivo fertility of non-sorted (NS) and sex-sorted (SS) semen. Thus, the aim was to correlate in vitro bull sperm functional parameters (experiment 1) and seminal plasma composition (experiment 2) with pregnancy rates using 2 cohorts of bulls (NS and SS). Experiment 1: ejaculates from each bull (n = 3 ejaculates per bull; n = 6 bulls for both NS and SS) were assessed for motility, thermal stress tolerance and morphology using microscopy, and viability, osmotic resistance, mitochondrial membrane potential, and acrosome integrity using flow cytometry. Fertilizing ability was assessed using IVF. Experiment 2: ejaculates (n = 3 per bull; n = 8 and 6 bulls for NS and SS, respectively) were collected, seminal plasma harvested and frozen and later analyzed for amino acid and fatty acid composition using gas chromatography mass spectrometry. In the NS cohort of bulls, there was no correlation between pregnancy rate and any of the sperm functional parameters assessed. However, within the SS cohort, motility and viability were correlated with pregnancy rate (r = 0.84 and 0.80, respectively; P < 0.05). There was no correlation between IVF outcome and pregnancy rate in either the SS or NS cohort of bulls. In the NS cohort of bulls, concentrations of the amino acid isoleucine and the fatty acid tricosylic acid (C23:0) were correlated with pregnancy rate (r = 0.80 and 0.74, respectively; P < 0.05). Within the SS cohort of bulls, the amino acid glutamic acid and the fatty acid arachidic acid (C20:0) were correlated with pregnancy rate (r = 0.84 and 0.82, respectively; P < 0.05). In conclusion, this study suggests that different in vitro markers of fertility are required to predict the fertility of NS and SS sperm.
Collections Ireland -> Teagasc -> PubMed

Full list of authors on original publication

S Fair, P Lonergan, S T Butler, L Brennan, A O'Gorman, H Whelan, C Murphy, B Fernandez-Fuertes, S A Holden

Experts in our system

1
Seán Fair
University of Limerick
Total Publications: 54
 
2
P. Lonergan
University College Dublin
Total Publications: 190
 
3
S T Butler
Teagasc
Total Publications: 81
 
4
Lorraine Brennan
University College Dublin
Total Publications: 143
 
5
Aoife O'Gorman
University College Dublin
Total Publications: 11
 
6
C Murphy
University of Limerick
Total Publications: 12
 
7
Beatriz Fernandez-Fuertes
University College Dublin
Total Publications: 8
 
8
Shauna A Holden
University of Limerick
Total Publications: 13