Type

Journal Article

Authors

Michel Simonneau
François Treussart
Huan-Cheng Chang
Bernadette Allinquant
Aiden Corvin
Carlo Sala
Yann Herault
Brigitte Potier
Chih-Che Wu
Feng-Jen Hsieh
and 13 others

Subjects

Veterinary

Topics
transport genetic disease risk brain diseases brain tracking risk factors abnormalities

Fluorescent nanodiamond tracking reveals intraneuronal transport abnormalities induced by brain-disease-related genetic risk factors. (2015)

Abstract Brain diseases such as autism and Alzheimer's disease (each inflicting >1% of the world population) involve a large network of genes displaying subtle changes in their expression. Abnormalities in intraneuronal transport have been linked to genetic risk factors found in patients, suggesting the relevance of measuring this key biological process. However, current techniques are not sensitive enough to detect minor abnormalities. Here we report a sensitive method to measure the changes in intraneuronal transport induced by brain-disease-related genetic risk factors using fluorescent nanodiamonds (FNDs). We show that the high brightness, photostability and absence of cytotoxicity allow FNDs to be tracked inside the branches of dissociated neurons with a spatial resolution of 12 nm and a temporal resolution of 50 ms. As proof of principle, we applied the FND tracking assay on two transgenic mouse lines that mimic the slight changes in protein concentration (∼30%) found in the brains of patients. In both cases, we show that the FND assay is sufficiently sensitive to detect these changes.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

Michel Simonneau, François Treussart, Huan-Cheng Chang, Bernadette Allinquant, Aiden Corvin, Carlo Sala, Yann Herault, Brigitte Potier, Chih-Che Wu, Feng-Jen Hsieh and 13 others

Experts in our system

1
Aiden Corvin
Trinity College Dublin
Total Publications: 190