Type

Journal Article

Authors

Gerard Cagney
Adrian P Bracken
John Crean
Kieran Wynne
Nayla Munawar
Benjamin Doyle
Darrell Andrews
Emilia Jerman
Gundula Streubel
Ariane Waston
and 2 others

Subjects

Biochemistry

Topics
polycomb repressive complex 2 regulation proteomics cells differentiation gene repression carcinoma cells proteins

Dynamic Protein Interactions of the Polycomb Repressive Complex 2 during Differentiation of Pluripotent Cells. (2016)

Abstract Polycomb proteins assemble to form complexes with important roles in epigenetic regulation. The Polycomb Repressive Complex 2 (PRC2) modulates the di- and tri-methylation of lysine 27 on histone H3, each of which are associated with gene repression. Although three subunits, EZH1/2, SUZ12, and EED, form the catalytic core of PRC2, a wider group of proteins associate with low stoichiometry. This raises the question of whether dynamic variation of the PRC2 interactome results in alternative forms of the complex during differentiation. Here we compared the physical interactions of PRC2 in undifferentiated and differentiated states of NTERA2 pluripotent embryonic carcinoma cells. Label-free quantitative proteomics was used to assess endogenous immunoprecipitation of the EZH2 and SUZ12 subunits of PRC2. A high stringency data set reflecting the endogenous state of PRC2 was produced that included all previously reported core and associated PRC2 components, and several novel interacting proteins. Comparison of the interactomes obtained in undifferentiated and differentiated cells revealed candidate proteins that were enriched in complexes isolated from one of the two states. For example, SALL4 and ZNF281 associate with PRC2 in pluripotent cells, whereas PCL1 and SMAD3 preferentially associate with PRC2 in differentiating cells. Analysis of the mRNA and protein levels of these factors revealed that their association with PRC2 correlated with their cell state-specific expression. Taken together, we propose that dynamic changes to the PRC2 interactome during differentiation may contribute to directing its activity during cell fate transitions.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

Gerard Cagney, Adrian P Bracken, John Crean, Kieran Wynne, Nayla Munawar, Benjamin Doyle, Darrell Andrews, Emilia Jerman, Gundula Streubel, Ariane Waston and 2 others

Experts in our system

1
Gerard Cagney
Royal College of Surgeons in Ireland
Total Publications: 54
 
2
Adrian Bracken
Trinity College Dublin
Total Publications: 25
 
3
John Crean
University College Dublin
Total Publications: 23
 
4
Kieran Wynne
Royal College of Surgeons in Ireland
Total Publications: 45
 
5
Nayla Munawar
Trinity College Dublin
Total Publications: 4
 
6
Emilia Jerman
Trinity College Dublin
Total Publications: 7
 
7
Gundula Streubel
Trinity College Dublin
Total Publications: 11