Journal Article


Brian A Lawlor
Charlotte E Teunissen
Henrik Zetterberg
Anders Skinningsrud
Maritta Siloaho
Brit Mollenhauer
Niels Kruse
Marleen Ja Koel-Simmelink
Sanna-Kaisa Herukka
Justyna M Bahl
and 2 others



neurodegenerative disease neurodegeneration validation cerebrospinal fluid neurofilament biomarker immunoassay diagnosis

Multicenter immunoassay validation of cerebrospinal fluid neurofilament light: a biomarker for neurodegeneration. (2016)

Abstract Neurofilament light (NfL) chain, a putative cerebrospinal fluid biomarker, can support neurodegenerative disease diagnosis and indicate disease severity and prognosis. Universal validation protocols when used to measure biomarkers can reduce pre and analytical laboratory variation, thus increasing end-user confidence in the consistency of validation data across sites. Here, a commercially available NfL ELISA (UmanDiagnostics, UmeƄ, Sweden) was validated in a multicentered setting using comprehensive newly developed standard operating procedures. The data showed good assay sensitivity and intra and interassay precision. Interlaboratory precision was, however, suboptimal. The UmanDiagnostics assay is suitable for the quantification of NfL in human cerebrospinal fluid. However, sources of interlaboratory variation in the data require further investigation.
Collections Ireland -> Trinity College Dublin -> PubMed

Full list of authors on original publication

Brian A Lawlor, Charlotte E Teunissen, Henrik Zetterberg, Anders Skinningsrud, Maritta Siloaho, Brit Mollenhauer, Niels Kruse, Marleen Ja Koel-Simmelink, Sanna-Kaisa Herukka, Justyna M Bahl and 2 others

Experts in our system

Brian A. Lawlor
National College Ireland
Total Publications: 125
Henrik Zetterberg
Trinity College Dublin
Total Publications: 9