Type

Journal Article

Authors

Marco Gobbetti
Douwe van Sinderen
Maria de Angelis
Alessia Lanera
Jennifer Mahony
Raffaella Di Cagno
Erica Pontonio

Subjects

Agriculture & Food Science

Topics
bacterial cell density 16s rrna sensory quality authentication lactic acid bacteria quantitative lactic acid

Sourdough authentication: quantitative PCR to detect the lactic acid bacterial microbiota in breads. (2016)

Abstract No national legislation anywhere in the world regulates and protects traditional/typical sourdough breads. Sourdough fermentation is firmly associated with a century-old tradition, and with sensory and nutritional quality of breads. A well-defined cell density of lactic acid bacteria has to be reached at the end of fermentation, and be indirectly detectable in baked breads. A Quantitative PCR (qPCR) method was developed to discriminate between breads made with and without sourdoughs. Universal primers targeting an approximately 178-bp fragment of the 16S rRNA-encoding gene of lactic acid bacteria were designed, covering the known diversity of sourdough lactic acid bacteria and excluding commonly encountered flour bacterial contaminants. A total of 191 breads either made with traditional type I and dried sourdough and baker's yeast, or by a chemical leavening method were shown to be accurately discriminated by means of qPCR. Discriminating values of gene copy number were only weakly correlated with pH values, and with lactate and acetate concentration, thus questioning the validity of these latter indirect indices. The use of sourdough has to be guaranteed to meet both bakery and consumer expectations, and to fulfil legal requirements; our work presents a reliable authentication method providing a suitable tool to satisfy such requirements.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Marco Gobbetti, Douwe van Sinderen, Maria de Angelis, Alessia Lanera, Jennifer Mahony, Raffaella Di Cagno, Erica Pontonio

Experts in our system

1
Marco Gobbetti
University College Cork
 
2
Douwe van Sinderen
University College Cork
Total Publications: 144
 
3
Maria de Angelis
University College Cork
 
4
Jennifer Mahony
University College Cork
Total Publications: 87
 
5
Raffaella Di Cagno
University College Cork
 
6
Erica Pontonio
University College Cork