Type

Journal Article

Authors

Douwe van Sinderen
Christian Cambillau
Thijs R. Kouwen
Marco Ventura
Horst Neve
Gabriele Andrea Lugli
Laurens Hanemaaijer
Barry Collins
Joana Oliveira
Jennifer Mahony

Subjects

Biochemistry

Topics
characterisation genetic interactions phage lactococcus lactis functional characterization architecture genomic analysis protein binding

Genetic and functional characterisation of the lactococcal P335 phage-host interactions. (2016)

Abstract Despite continuous research efforts, bacterio(phages) infecting Lactococcus lactis starter strains persist as a major threat to dairy fermentations. The lactococcal P335 phages, which are currently classified into four sub-groups (I-IV), are the second most frequently isolated phage group in an industrial dairy context. The current work describes the isolation and comparative genomic analysis of 17 novel P335 group phages. Detailed analysis of the genomic region of P335 phages encoding the so-called "baseplate", which includes the receptor binding protein (RBP) was combined with a functional characterization of the RBP of sub-group III and IV phages. Additionally, calcium-dependence assays revealed a specific requirement for calcium by sub-group IV phages while host range analysis highlighted a higher number of strains with CWPS type A (11 of 39 strains) are infected by the P335 phages assessed in this study than those with a C (five strains), B (three of 39 strains) or unknown (one of 39 strains) CWPS type. These analyses revealed significant divergence among RBP sequences, apparently reflecting their unique interactions with the host and particularly for strains with a type A CWPS. The implications of the genomic architecture of lactococcal P335 phages on serving as a general model for Siphoviridae phages are discussed.
Collections Ireland -> University College Cork -> PubMed

Full list of authors on original publication

Douwe van Sinderen, Christian Cambillau, Thijs R. Kouwen, Marco Ventura, Horst Neve, Gabriele Andrea Lugli, Laurens Hanemaaijer, Barry Collins, Joana Oliveira, Jennifer Mahony

Experts in our system

1
Douwe van Sinderen
University College Cork
 
2
Christian Cambillau
University College Cork
Total Publications: 15
 
3
Thijs R. Kouwen
University College Cork
Total Publications: 9
 
4
Marco Ventura
University College Cork
Total Publications: 31
 
5
Horst Neve
University College Cork
Total Publications: 31
 
6
Gabriele A. Lugli
University College Cork
Total Publications: 17
 
7
Laurens Hanemaaijer
University College Cork
Total Publications: 9
 
8
Barry Collins
University College Cork
Total Publications: 11
 
9
Joana Oliveira
University College Cork
Total Publications: 6
 
10
Jennifer Mahony
University College Cork
Total Publications: 71