Type

Journal Article

Authors

Stephen R Pennington
William M Gallagher
Ning Tang
Peter Stone
Ben C Collins
Thomas Y K Lau

Subjects

Chemistry

Topics
generation mass spectrometry toxicity quantification proteins technology toxicological biomarkers

Absolute Quantification of Toxicological Biomarkers via Mass Spectrometry. (2017)

Abstract With the advent of "-omics" technologies there has been an explosion of data generation in the field of toxicology, as well as many others. As new candidate biomarkers of toxicity are being regularly discovered, the next challenge is to validate these observations in a targeted manner. Traditionally, these validation experiments have been conducted using antibody-based technologies such as Western blotting, ELISA, and immunohistochemistry. However, this often produces a significant bottleneck as the time, cost, and development of successful antibodies are often far outpaced by the generation of targets of interest. In response to this, there recently have been several developments in the use of triple quadrupole (QQQ) mass spectrometry (MS) as a platform to provide quantification of proteins. This technology does not require antibodies; it is typically less expensive and quicker to develop assays and has the opportunity for more accessible multiplexing. The speed of these experiments combined with their flexibility and ability to multiplex assays makes the technique a valuable strategy to validate biomarker discovery.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Stephen R Pennington, William M Gallagher, Ning Tang, Peter Stone, Ben C Collins, Thomas Y K Lau

Experts in our system

1
Stephen R Pennington
University College Dublin
 
2
William M Gallagher
University College Dublin
Total Publications: 148
 
3
Ben C Collins
University College Dublin
Total Publications: 8
 
4
Thomas Y K Lau
University College Dublin
Total Publications: 7