Type

Journal Article

Authors

E Gormley
S J More
A Duignan
M Doyle
M Good
T A Clegg

Subjects

Veterinary

Topics
test performance post mortem diagnostic tests diagnostic testing quality assurance interferon ifn mycobacterium bovis assay performance

The performance of the interferon gamma assay when used as a diagnostic or quality assurance test in Mycobacterium bovis infected herds. (2016)

Abstract There are two different contexts in the Irish bTB eradication programme in which the interferon-gamma assay (IFN-γ) is applied. Firstly, the IFN-γ assay is applied routinely to high risk cohorts in herds with four or more reactors to the SICTT. The IFN-γ test is then carried out on blood samples submitted to the laboratory within 8h of collection (diagnostic testing). Secondly, the use of the IFN-γ assay has recently been extended to test SICTT reactors as part of a general quality assurance (QA) scheme to monitor the performance of the SICTT. Blood samples from reactors are tested one day after blood collection (QA testing). In this study, we analysed the relative performance of the SICTT and IFN-γ when used in parallel as an 8h diagnostic test and as a 24h QA test on SICTT reactors. A total of 17,725 IFN-γ tests were included in the analysis (11,658 diagnostic tests and 6067 QA tests). Of the samples submitted for diagnostic testing, the proportion positive to IFN-γ decreased with the severity of interpretation of the SICTT result. Of the standard reactors that were tested with IFN-γ in the QA programme, 92.2% were positive to the IFN-γ test. Among animals that were SICTT -ve/IFN-γ +ve, 18.9% were positive at post-mortem compared to 11.8% of those that were SICTT +ve (standard reactor)/IFN-γ -ve. These results highlight the risk associated with retaining SICTT -ve/IFN-γ +ve animals, and suggest that prompt removal of these animals is necessary to reduce the potential for future transmission.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

E Gormley, S J More, A Duignan, M Doyle, M Good, T A Clegg

Experts in our system

1
E Gormley
University College Dublin
Total Publications: 67
 
2
S J More
University College Dublin
Total Publications: 171
 
3
A Duignan
University College Dublin
Total Publications: 7
 
4
Mairead B Doyle
University College Dublin
Total Publications: 8
 
5
M Good
University College Dublin
Total Publications: 28
 
6
T A Clegg
University College Dublin
Total Publications: 46