Type

Journal Article

Authors

J Alfredo Martinez
John C Mathers
Michael J Gibney
Eileen R Gibney
Yannis Manios
Iwonna Traczyk
Wim H Saris
Hannelore Daniel
Julie A Lovegrove
Lorraine Brennan
and 7 others

Subjects

Medicine & Nursing

Topics
standard deviation gene oral primary healthcare blood cells methylation public health practice white blood cells

Gene methylation parallelisms between peripheral blood cells and oral mucosa samples in relation to overweight. (2016)

Abstract Epigenetics has an important role in the regulation of metabolic adaptation to environmental modifications. In this sense, the determination of epigenetic changes in non-invasive samples during the development of metabolic diseases could play an important role in the procedures in primary healthcare practice. To help translate the knowledge of epigenetics to public health practice, the present study aims to explore the parallelism of methylation levels between white blood cells and buccal samples in relation to obesity and associated disorders. Blood and buccal swap samples were collected from a subsample of the Spanish cohort of the Food4Me study. Infinium HumanMethylation450 DNA Analysis was carried out for the determination of methylation levels. Standard deviation for β values method and concordance correlation analysis were used to select those CpG which showed best parallelism between samples. A total of 277 CpGs met the criteria and were selected for an enrichment analysis and a correlation analysis with anthropometrical and clinical parameters. From those selected CpGs, four presented high associations with BMI (cg01055691 in GAP43; r = -0.92 and rho = -0.84 for blood; r = -0.89 and rho = -0.83 for buccal sample), HOMA-IR (cg00095677 in ATP2A3; r = 0.82 and rho = -0.84 for blood; r = -0.8 and rho = -0.83 for buccal sample) and leptin (cg14464133 in ADARB2; r = -0.9182 and rho = -0.94 for blood; r = -0.893 and rho = -0.79 for buccal sample). These findings demonstrate the potential application of non-invasive buccal samples in the identification of surrogate epigenetic biomarkers and identify methylation sites in GAP43, ATP2A3 and ADARB2 genes as potential targets in relation to overweight management and insulin sensibility.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

J Alfredo Martinez, John C Mathers, Michael J Gibney, Eileen R Gibney, Yannis Manios, Iwonna Traczyk, Wim H Saris, Hannelore Daniel, Julie A Lovegrove, Lorraine Brennan and 7 others

Experts in our system

1
J Alfredo Martinez
University College Dublin
Total Publications: 32
 
2
John C Mathers
University College Dublin
Total Publications: 33
 
3
Michael J Gibney
University College Dublin
Total Publications: 102
 
4
Eileen R Gibney
University College Dublin
Total Publications: 98
 
5
Yannis Manios
University College Dublin
Total Publications: 38
 
6
Wim H M Saris
University College Dublin
Total Publications: 39
 
7
Hannelore Daniel
University College Dublin
Total Publications: 33
 
8
Julie A Lovegrove
University College Dublin
Total Publications: 46
 
9
Lorraine Brennan
University College Dublin
Total Publications: 166