Type

Journal Article

Authors

Ross C. Ethier
Daniel J Kelly
Manu O. Platt
Irsham Zaman
Lisa A. Schildmeyer
Eric J. Snider
Julia Raykin
C Ross Ethier
John J. Mulvihill

Subjects

Biochemistry

Topics
risk factor glaucoma 3d culture model optic nerve head optic nerve mechanical conditioning astrocyte mechanobiology cell death compression 3d collagen gels collagen gels intraocular pressure astrocytes

Development of a platform for studying 3D astrocyte mechanobiology: compression of astrocytes in collagen gels (2017)

Abstract The full text of this article will not be available in ULIR until the embargo expires on the 27/11/2018 Glaucoma is a common optic neuropathy characterized by retinal ganglion cell death. Elevated intraocular pressure (IOP), a key risk factor for glaucoma, leads to significant biomechanical deformation of optic nerve head (ONH) cells and tissues. ONH astrocytes respond to this deformation by transforming to a reactive, proliferative phenotype, which has been implicated in the progression of glaucomatous vision loss. However, little is known about the mechanisms of this transformation. In this study, we developed a 3D collagen gel culture system to mimic features of ONH deformation due to elevated IOP. Compressive loading of astrocyte-seeded collagen gels led to cell alignment perpendicular to the direction of strain, and increased astrocyte activation, as assayed by GFAP, vimentin, and s100β levels, as well as MMP activity. This proof-of-concept study shows that this system has potential for studying mechanisms of astrocyte mechanobiology as related to the pathogenesis of glaucoma. Further work is needed to establish the possible interplay of mechanical stimulation, matrix properties, and hypoxia on the observed response of astrocytes.
Collections Ireland -> University of Limerick -> Faculty of Science and Engineering
Ireland -> University of Limerick -> Mechanical, Aeronautical & Biomedical Engineering
Ireland -> University of Limerick -> Departments Science and Engineering

Full list of authors on original publication

Ross C. Ethier, Daniel J Kelly, Manu O. Platt, Irsham Zaman, Lisa A. Schildmeyer, Eric J. Snider, Julia Raykin, C Ross Ethier, John J. Mulvihill

Experts in our system

1
Daniel Kelly
Trinity College Dublin
Total Publications: 168
 
2
John J Mulvihill
University of Limerick
Total Publications: 17