Type

Journal Article

Authors

David Kenny
Joseph Cassidy
Marijke Beltman
Sinead Waters
Eoin O’Hara
Keelan McNamara
Eoin O'Hara
Carla Surlis

Subjects

Veterinary

Topics
gene expression profiles mode of delivery calf caesarean section il 1 jejunum elective caesarean lung birth inflammatory response genes bovine real time pcr beef calves

Birth delivery method affects expression of immune genes in lung and jejunum tissue of neonatal beef calves (2017)

Abstract Background Caesarean section is a routine veterinary obstetrical procedure employed to alleviate dystocia in cattle. However, CS, particularly before the onset of labour, is known to negatively affect neonatal respiration and metabolic adaptation in humans, though there is little published information for cattle. The aim of this study was to investigate the effect of elective caesarean section (ECS) or normal trans-vaginal (TV) delivery, on lung and jejunal gene expression profiles of neonatal calves. Results Paternal half-sib Angus calves (gestation length 278 + 1.8 d) were delivered either transvaginally (TV; n = 8) or by elective caesarean section (ECS; n = 9) and immediately euthanized. Lung and jejunum epithelial tissue was isolated and snap frozen. Total RNA was extracted using Trizol reagent and reverse transcribed to generate cDNA. For lung tissue, primers were designed to target genes involved in immunity, surfactant production, cellular detoxification, membrane transport and mucin production. Primers for jejunum tissue were chosen to target mucin production, immunoglobulin uptake, cortisol reaction and membrane trafficking. Quantitative real-time PCR reactions were performed and data were statistically analysed using mixed models ANOVA. In lung tissue the expression of five genes were affected (p < 0.05) by delivery method. Four of these genes were present at lower (LAP, CYP1A1, SCN11α and SCN11β) and one (MUC5AC) at higher abundance in ECS compared with TV calves. In jejunal tissue, expression of TNFα, Il-1β and 1 l-6 was higher in ECS compared with TV calves. Conclusions This novel study shows that ECS delivery affects the expression of key genes involved in the efficiency of the pulmonary liquid to air transition at birth, and may lead to an increased inflammatory response in jejunal tissue, which could compromise colostral immunoglobulin absorption. These findings are important to our understanding of the viability and management of neonatal calves born through ECS.
Collections Ireland -> Teagasc -> Teagasc publications in Biomed Central
Ireland -> Teagasc -> Other Teagasc Research

Full list of authors on original publication

David Kenny, Joseph Cassidy, Marijke Beltman, Sinead Waters, Eoin O’Hara, Keelan McNamara, Eoin O'Hara, Carla Surlis

Experts in our system

1
D.A. Kenny
Teagasc
Total Publications: 147
 
2
Joseph P. Cassidy
University College Dublin
Total Publications: 55
 
3
Marijke Eileen Beltman
University College Dublin
Total Publications: 24
 
4
Sinead M. Waters
Teagasc
Total Publications: 99
 
5
Eoin O'Hara
Teagasc
Total Publications: 4
 
6
Carla Surlis
Maynooth University
Total Publications: 11