Type

Journal Article

Authors

Michael A. Morris
Joseph P Kerry
Malco Cruz-Romero
Suresh C. Pillai
Sibu C. Padmanabhan
Mary Garvey

Subjects

Microbiology

Topics
zeta potential cytotoxicity cell lines cell membrane silver nanoparticles food packaging skin cells lung cell

In vitro cytotoxicity of water soluble silver (Ag) nanoparticles on HaCat and A549 cell lines (2017)

Abstract The wide range of applications of silver nanoparticles (AgNPs) in commercial products, including food packaging, has encouraged researchers to come up with novel preparation methods for the production of these robust materials. The methods resulting in the formation of NPs for such commercial applications clearly demand a good accounting of their toxicity aspects to humans as well as the environment. We herein present a chemical preparation method for the production of size- and shape-defined AgNPs and investigate the impact of these nanoparticles on HaCat and A549 cell lines. Findings show that lung cells (A549) are more sensitive than skin cells (HaCat) to Ag induced toxicity, evident by the significantly (p<0.05) reduced LC50 for all NPs under study. The current investigation showed that the extent of surface capping agent (citrate) and size influenced the cell toxicity, where a lesser surface coverage (zeta potential, ζ, -27.7 mV) and smaller size (~17 nm) enhanced the toxicity compared to comparatively bigger particles (~39 nm) with higher surface coverage (ζ, -47.3 mV). The size- and shape-defined particles such as triangles which have proven useful for many applications, due to their high energy/high field edges, were found to be less toxic against biological cell lines and therefore may have potential to be used in food packaging applications as reservoirs of silver ions. A striking difference in cell line toxicity within such a small size window clearly demonstrates the vital roles played by the smaller size, difference in shape and lesser surface coverage in defining a higher passive cell membrane diffusion followed by silver dissolution inside cell cytoplasm increasing cytotoxicity.
Collections Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> Food and Nutritional Sciences - Journal Articles
Ireland -> University College Cork -> Food and Nutritional Sciences
Ireland -> University College Cork -> Chemistry
Ireland -> University College Cork -> Chemistry - Journal Articles

Full list of authors on original publication

Michael A. Morris, Joseph P Kerry, Malco Cruz-Romero, Suresh C. Pillai, Sibu C. Padmanabhan, Mary Garvey

Experts in our system

1
Michael A. Morris
University College Cork
Total Publications: 121
 
2
J P Kerry
University College Cork
Total Publications: 130
 
3
Malco Cruz-Romero
University College Cork
Total Publications: 7
 
4
Suresh Pillai
Dublin Institute of Technology
Total Publications: 70