Type

Journal Article

Authors

Douwe van Sinderen
John MacSharry
Horst Neve
Thijs R. Kouwen
Laurens Hanemaaijer
Jennifer Mahony
Joana Oliveira

Subjects

Veterinary

Topics
electron microscopy lysogeny bacteriophage populations temperate phages tp901 1 marine viruses temperate chemical inductions pcr strains prophage phage flow cytometry

Detecting Lactococcus lactis prophages by Mitomycin C-mediated induction coupled to flow cytometry analysis (2017)

Abstract Most analyzed Lactococcus lactis strains are predicted to harbor one or more prophage genomes within their chromosome; however, the true extent of the inducibility and functionality of such prophages cannot easily be deduced from sequence analysis alone. Chemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to measure the number of released phage particles. Here, we report the application of flow cytometry as a reliable tool for the detection and enumeration of released lactococcal prophages using the green dye SYTO-9.
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Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
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Full list of authors on original publication

Douwe van Sinderen, John MacSharry, Horst Neve, Thijs R. Kouwen, Laurens Hanemaaijer, Jennifer Mahony, Joana Oliveira

Experts in our system

1
Douwe van Sinderen
University College Cork
Total Publications: 144
 
2
John MacSharry
University College Cork
Total Publications: 40
 
3
Horst Neve
University College Cork
Total Publications: 41
 
4
Thijs R. Kouwen
University College Cork
Total Publications: 10
 
5
Laurens Hanemaaijer
University College Cork
Total Publications: 10
 
6
Jennifer Mahony
University College Cork
Total Publications: 87
 
7
Joana Oliveira
University College Cork
Total Publications: 7