Type

Journal Article

Authors

Marco Ventura
Douwe van Sinderen
Tiziana Meschi
Antonio Nouvenne
Andrea Ticinesi
Alice Viappiani
Marta Mangifesta
Francesca Turroni
Gabriele A Lugli
Christian Milani
and 1 others

Subjects

Microbiology

Topics
metagenomics fatty acid lipid metabolism functionality hydrogen production composition constipation gut microbiota

Unveiling the gut microbiota composition and functionality associated with constipation through metagenomic analyses (2017)

Abstract Functional constipation (FC) is a gastrointestinal disorder with a high prevalence among the general population. The precise causes of FC are still unknown and are most likely multifactorial. Growing evidence indicates that alterations of gut microbiota composition contribute to constipation symptoms. Nevertheless, many discrepancies exist in literature and no clear link between FC and gut microbiota composition has as yet been identified. In this study, we performed 16 S rRNA-based microbial profiling analysis of 147 stool samples from 68 FC individuals and compared their microbial profiles with those of 79 healthy subjects (HS). Notably, the gut microbiota of FC individuals was shown to be depleted of members belonging to Bacteroides, Roseburia and Coprococcus 3. Furthermore, the metabolic capabilities of the gut microbiomes of five FC and five HS individuals were evaluated through shotgun metagenomics using a MiSeq platform, indicating that HS are enriched in pathways involved in carbohydrate, fatty acid and lipid metabolism as compared to FC. In contrast, the microbiomes corresponding to FC were shown to exhibit high abundance of genes involved in hydrogen production, methanogenesis and glycerol degradation. The identified differences in bacterial composition and metabolic capabilities may play an important role in development of FC symptoms.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Marco Ventura, Douwe van Sinderen, Tiziana Meschi, Antonio Nouvenne, Andrea Ticinesi, Alice Viappiani, Marta Mangifesta, Francesca Turroni, Gabriele A Lugli, Christian Milani and 1 others

Experts in our system

1
Marco Ventura
University College Cork
Total Publications: 31
 
2
Douwe van Sinderen
University College Cork
 
3
Marta Mangifesta
University College Cork
Total Publications: 3
 
4
Francesca Turroni
University College Cork
Total Publications: 8
 
5
Gabriele Andrea Lugli
University College Cork
Total Publications: 17
 
6
Christian Milani
University College Cork
Total Publications: 9