Type

Journal Article

Authors

Cormac G. Gahan
Mark Tangney
Kevin P Francis
Colin Hill
Giuliano Bensi
Angela Spagnuolo
Pat G Casey
Michael Stanton
Sadeeq Ur Rahman

Subjects

Microbiology

Topics
click beetle luciferase virulence clinical bioluminescence cell culture pathogen listeria monocytogenes in vivo imaging

Development of a click beetle luciferase reporter system for enhanced bioluminescence imaging of Listeria monocytogenes: analysis in cell culture and murine infection models (2017)

Abstract Listeria monocytogenes is a Gram-positive facultative intracellular pathogen that is widely used as a model organism for the analysis of infection biology. In this context, there is a current need to develop improved reporters for enhanced bioluminescence imaging (BLI) of the pathogen in infection models. We have developed a click beetle red luciferase (CBR-luc) based vector (pPL2CBRopt) expressing codon optimized CBR-luc under the control of a highly expressed Listerial promoter (PHELP) for L. monocytogenes and have compared this to a lux-based system expressing bacterial luciferase for BLI of the pathogen using in vitro growth experiments and in vivo models. The CBR-luc plasmid stably integrates into the L. monocytogenes chromosome and can be used to label field isolates and laboratory strains of the pathogen. Growth experiments revealed that CBR-luc labeled L. monocytogenes emits a bright signal in exponential phase that is maintained during stationary phase. In contrast, lux-labeled bacteria produced a light signal that peaked during exponential phase and was significantly reduced during stationary phase. Light from CBR-luc labeled bacteria was more efficient than the signal from lux-labeled bacteria in penetrating an artificial tissue depth assay system. A cell invasion assay using C2Bbe1 cells and a systemic murine infection model revealed that CBR-luc is suited to BLI approaches and demonstrated enhanced sensitivity relative to lux in the context of Listeria infection models. Overall, we demonstrate that this novel CBR reporter system provides efficient, red-shifted light production relative to lux and may have significant applications in the analysis of L. monocytogenes pathogenesis
Collections Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Cormac G. Gahan, Mark Tangney, Kevin P Francis, Colin Hill, Giuliano Bensi, Angela Spagnuolo, Pat G Casey, Michael Stanton, Sadeeq Ur Rahman

Experts in our system

1
Cormac G M Gahan
University College Cork
Total Publications: 109
 
2
Mark Tangney
University College Cork
Total Publications: 53
 
3
Kevin P. Francis
University College Cork
Total Publications: 9
 
4
Colin Hill
University College Cork
Total Publications: 351
 
5
Pat G. Casey
University College Cork
Total Publications: 40
 
6
Michael Stanton
University College Cork
Total Publications: 6